|Land Without Magic|
|The Queen is Dead|
Initially appearing as the sweet and kind fiancée of Neal Cassidy, it is soon revealed that she is actually working for "the Home Office" to destroy all magic. She was the main antagonist for the second half of Season 2, along with Greg.
Season 2 (6/22)
- The Queen is Dead
- Selfless, Brave and True
- The Evil Queen
- Second Star to the Right
- And Straight On 'Til Morning
Season 3 (1/22)
- Along with Rumplestiltskin, Emma Swan and Nimue, Tamara and Greg are the only antagonists not to be from a Disney work.
- Regina Mills was the main antagonist of "Snow White and the Seven Drawfs".
- Cora Mills was the main antagonist of "Alice in Wonderland".
- Peter Pan was a protagonist in the film of the same name.
- Zelena was the main antagonist of "The Wizard of Oz", however, Disney produced a prequel to this film in which she appears named Theodora.
- Ingrid was the main antagonist and deuteragonist in "Frozen" as Elsa. In Once Upon a Time, they are two different characters but in Disney they are one character known as Elsa the Snow Queen.
- Cruella, Ursula and Maleficent are all main antagonists of separate Disney films.
- Arthur was a protagonist in "The Sword in the Stone".
- Hades was the main antagonist of "Hercules".